Figure 6

Effect of in vivo treatment with EGCG on the activation of NF-κB, IKK activation, and IκB-α degradation in rats subjected to myocardial ischemia and reperfusion. EGCG-treated rats received a bolus dose of EGCG 10 mg/kg at the end of the ischemia period followed by 10 mg/kg/h intravenous infusion for the entire period of reperfusion. A: Representative autoradiograph of electrophoretic mobility shift assay for NF-κB binding hearts of vehicle-treated rats at 0 (sham value), 30, 60, and 120 min after reperfusion. B: Representative Western blot analysis demonstrating the effect of vehicle or EGCG on IκB-α degradation at 0 (sham value), 30, 60, and 120 min after reperfusion. Western blot analysis is representative of 3 separate experiments performed at different times. Fold increase was calculated compared with respective sham value (time 0) set to 1.0. Results are representative of 3 separate time-course experiments. *P < 0.05 compared with respective sham value (time 0); #P < 0.05 compared with vehicle-treated rats subjected to myocardial ischemia and reperfusion. C: Representative of autoradiograph of kinase assay demonstrating the effect of EGCG or vehicle treatment on activation of the IKK complex at 0 (sham value), 30, 60, and 120 min after reperfusion. The autoradiograph is representative of 3 experiments with similar results. Fold increase was calculated compared with respective sham value (time 0) set to 1.0. Results are representative of 3 separate time-course experiments. *P < 0.05 compared with respective sham value (time 0); #P < 0.05 versus vehicle-treated rats subjected to myocardial ischemia and reperfusion.