Figure 4
Effect of hemozoin and β-hematin on COX-2 protein expression. PBMCs (1×106 cells/mL) were stimulated with media alone, LPS (100 ng/mL) and IFN-γ (200 U/mL), and LPS and IFN-γ in the presence of hemozoin (10, 1.0, and 0.1 γg/mL) or β-hematin (10, 1.0, and 0.1 γg/mL). A,C: Cells were harvested at 48 h for COX-2 protein determination by immunoblot analysis. Shown is a representative immunoblot of 4 independent experiments in different individuals for cells treated with (A) hemozoin or (C) β-hematin. As a positive control (+), extracts of mouse macrophage cells (RAW 264.7), stimulated with lipopolysaccharide (E. coli LPS 0127B8, 1.0 µg/mL) and IFN-γ (10 ng/mL) were included. B,D: COX-2 antigen expression was quantified using commercially available software (Scion Image, Scion Corporation) in cells treated with (B) hemozoin or (D) β-hematin and expressed as relative arbitrary units.