Figure 7
Effect of endogenous IL-10 on hemozoin-induced PGE2 suppression. A: PBMCs (1 × 106 cells/mL) were stimulated with media alone, LPS (100 ng/mL) and IFN-y (200 U/mL), and LPS and IFN-y in the presence of hemozoin (10 µg/mL), and supernatants were obtained at 48 h for IL-10 determination. Values are the mean ± SEM for 10 individuals, n = 4/condition for each individual. Statistical significance was determined by the Mann-Whitney U test. *P < 0.01 compared with control (media alone); **P < 0.05 compared with stimulated conditions. B,C: PBMCs (1 × 106 cells/mL) were stimulated with media alone, LPS (100 ng/mL) and IFN-γ (200 U/mL), and LPS and IFN-γ in the presence of hemozoin (10 µg/mL, solid black line), and were treated with varying concentrations of an IL-10 neutralizing antibody (1.0, 0.5, and 0.1 µg/mL). Supernatants were obtained at 48 h for (B) PGE2 and (C) IL-10 determination. Values are the mean ± SEM, n = 5. Statistical significance was determined by the Mann-Whitney U test. *P < 0.01 compared with control (media alone); **P < 0.05 compared to stimulated conditions.