Figure 4

Imprinting status of Igf2 in infantile hemangioma. (A) Agarose gel electrophoresis of ApaI-digested PCR products of Igf2 exon 9 amplified from genomic DNA. Proliferating hemangioma designated 39, 55, 57, involuting hemangioma I-18, I-21, I-24, and normal infant skin F3 were heterozygous (A and B alleles). I-13 (B allele) and I-19 (A allele) were homozygous. The results are representative of 27 proliferating hemangiomas, 21 involuting hemangiomas, and 11 normal infant skin specimens (see Table 1). (B) Agarose gel electrophoresis of ApaI-digested cDNA products of Igf2. Monoallelic Igf2 expression is shown in proliferating hemangiomas 35, 38, 51, 53, 57, 63, involuting hemangiomas I-16, I-18, I-20, I-21, I-24, I-25, I-30, and normal infant skin control F3 and F10. The results are representative of the analysis of 15 informative cases. Homozygous hemangioma I-13 and skin control F4 and F11 were included to verify that ApaI digestions had gone to completion.