Figure 3
From: The Role of AEBP1 in Sex-Specific Diet-Induced Obesity
Increased survival signal in WAT of TG mice resulted in adipocyte hyperplasia. (A) The levels of phosphorylated Akt (p-AKT), total Akt (AKT), and phosphorylated Gsk-3β (p-GSK) in NT and TG mice were measured before (−) and after (+) insulin injection by immunoblotting with respective antibody. The average values of p-AKT (middle panel) and p-GSK (right panel) levels after normalized with AKT are represented as mean ± SEM. *P < 0.05 for NT (n = 5) and TG (n = 4), **P = 0.01 for NT and TG (n = 3). (B) The fat cell size, which was expressed in milligrams of lipid per cell, was measured by counting the total number of osmium tetroxide-fixed cells obtained from a known weight of tissue lipid. NT, n = 2; TG, n = 2. (C) The total number of adipocytes in 0.775 g mean weight of WAT from TG (n = 2) mice and 0.515 g mean weight of WAT from NT (n = 2) mice are shown in millions of cells. *P < 0.05. (D) WAT histology showing unilocular cells in paraffin sections stained with hematoxylin and eosin.