Figure 1

(a) Chemokine receptor expression after bone marrow reconstitution of irradiated mice. Expression of CCR1 transcripts and other CC chemokine receptors was followed by multiplex RT-PCR in splenocytes of LDLr^−/− mice reconstituted with bone marrow from either CCR1^−/− or control CCR1^+/+ mice. The data are representative of 3 mice in each group. (b) Representative photomicrograph showing specific CCL3 staining (red) in a macrophage-rich plaque from CCR1^−/− mice. Similar staining was observed in CCR1^+/+ plaques (not shown). (c) Representative positive staining (red) for CCR1 protein in macrophage-rich plaques obtained from CCR1^+/+ mice and its absence in plaques of CCR1^−/− mice. Original magnifications × 200 (b), × 100 (c).