Table 1 Primer sequences and RT-PCR amplification conditions.
Gene | Primer sequence | Annealing temperature, °C | No. of cycles | Product size, bp |
|---|---|---|---|---|
COL-I | 5′-TTGACCCTAACCAAGGATGC 5′-CACCCCTTCTGCGTTGTATT | 54 | 30 | 197 |
COL-III | 5′-AGGCCAATGGCAATGTAAAG 5′-GGCCTTGCGTGTTTGATATT | 53 | 30 | 187 |
TGF-β1 | 5′-CACCTGCACAGCTCCAGGCAC 5′-CTTGCGACCCACGTAGTAGACG | 54 | 32 | 153 |
HGF | 5′-TCGTTCCTTGGGATTATTGC 5′-TGGTGCTGACTGCATTTCTC | 55 | 32 | 571 |
TIMP-1 | 5′-TCCCCAGAAATCATCGAGAC 5′-ATGGCTGAACAGGGAAACAC | 55 | 32 | 329 |
LH2b | 5′-TGCGATCAGAGATGAGTGAA 5′-GTTGAGATGGGAAGTGTTGTAAT | 53 | 32 | 229 |
E-cadherin | 5′-GCAGTTCTGCCAGAGAAACC 5′- AATCCTGCTTCCAGGGAGAT | 55 | 28 | 315 |
N-cadherin | 5′-TGTTGCTGCAGAAAACCAAG 5′-GGCGACTCTCTGTCCAGAAC | 53 | 28 | 309 |
DSG-2 | 5′-CCTTGGTGGACCAGCACTAT 5′-GACAGCACCGTCTTCCTAGC | 57 | 35 | 316 |
DSC-2 | 5′-CGTAATTCCCGTCCGAGTTA 5′-TTGAGCTGTGAAGCCATTTG | 55 | 35 | 386 |
GAPDH | 5′-ATGGTGAAGGTCGGTGTGAAC 5′- GCTGACAATCTTGAGGGAGT | 54 | 28 | 473 |