Expression cassette of the FVII-GFP and localization of chimeras within living BHK cells. (A) Schematic representation of the FVII-GFP cassette. PP, FVII preproleader sequence; GFP, enhanced green fluorescence protein. (B-E): Fluorescence images of cells expressing Wt-FVII-GFP (B), PKCβ-GFP (C), −42PFVIIGFP (D), and −48PFVII-GFP (E) before (time 0) and 45 or 90 s after treatment with digitonin. For each construct, the cell shown is representative of at least 50 cells examined in 3 independent experiments. Graphics report the average cell fluorescence over time given as percentage of the fluorescence at time 0.