Figure 13From: The Histone Deacetylase Inhibitor ITF2357 Reduces Production of Pro-Inflammatory Cytokines In Vitro and Systemic Inflammation In VivoReal-time PCR of steady-state TNFα mRNA. Real-time PCR was performed on RNA extracted from resting PBMCs 4 h after stimulation with LPS (10 ng/mL) in the presence of 50 nM ITF2357. The data were calculated as mRNA in picograms per nanogram of 18S RNA. A value of 1.0 was assigned for the unstimulated control of each donor (indicated as A, B, and C), and the fold change was calculated for each donor.Back to article page