Figure 3

Effect of ITF2357 on intracellular IL-1 α and secreted IL-1 β from LPS-stimulated PBMCs. Freshly obtained PBMCs were preincubated for 1 h with increasing nanomolar concentrations of ITF2357 and then stimulated with LPS (10 ng/mL) for 24 hours. (A) Levels of intracellular IL-1 a are shown as the percent change from LPS only (set at 100%) as indicated on the vertical axis. (B) Levels of secreted IL-1 β are shown as the percent change from LPS only. The data are the mean percent change ± SEM of 6 donors. The dashed line indicates inhibition of 50%. (C) FACS analysis of percent of total cell PI staining expressed at sub-G₁ cell cycle. Mean ± SEM of 4 donors are shown. Cells in sub-G₁ cycle are from the same cultures shown in B. *P < 0.05; **P < 0.01 from LPS only.