Effect of ITF2357 on apoptosis and necrosis in monocytes. Purified monocytes in RPMI were incubated with increasing concentrations of ITF2357 for 1 h followed by the addition of LPS (10 ng/mL) to appropriate wells. After 24 h at 37° C, the percentage of annexin-V- and PI-positive cells was determined. (A) The data are the mean ± SEM of 3 separate experiments/donors. (B) FACS analysis of PI and annexin V staining in control monocytes. (C) PI and annexin V staining in monocytes incubated with 100 nM ITF2357. (D) PI and annexin V staining in monocytes incubated with 1000 nM ITF2357. The data in B, C, and D are from 1 representative experiment.