Figure 1

Unsupervised hierarchical cluster analysis of RNA from 9 RA and 13 control PBMC samples. Total RNA samples were analyzed on oligonucleotide arrays as described. In no case were samples pooled. Genes were selected for analysis if they had a present call, a frequency greater than 10 ppm, and two-fold change expression in five of nine RA samples. The expression patterns of 330 genes are displayed in a dendrogram where columns represent each sample and rows represent individual genes. Genes are colored on a gradient (from −10-fold to 10-fold), with those increase in expression relative to the average of the control in red. Those that decrease are in blue, and those with little or no change are in yellow. A, region where expression levels in the RA samples were increased compared with the normal samples.