Figure 1

Screening of HCC-specific peptides from 12-mer peptide libraries. (A) Output assay for each plaque selection round. The output ratio was calculated as output number/input number. (B) Binding assay of positive clones to the hepatoma cell lines (SMMC-7721 and BEL-7402) and to the normal liver cell line (HL-02). The binding capacity was quantified by phage ELISA assay. The data are presented as means of 5 positive phage clones, and the error bars indicate standard deviation. (C) Competitive binding of phage clones to SMMC-7721 with HAb25, which was diluted at the starting concentration (100 µg mL⁻¹). The inhibition rate was calculated as the signal of phage binding with versus without HAb25.