Skip to main content
Figure 3 | Molecular Medicine

Figure 3

From: Targeting of Hepatoma Cell and Suppression of Tumor Growth by a Novel 12mer Peptide Fused to Superantigen TSST-1

Figure 3

Characterization of the 12T fusion protein. (A) Purification of the 12T fusion protein. 12T was expressed in E. coli DH5α cells and analyzed by SDS-PAGE electrophoresis. Coomassie blue stain. Lanes 1–9 represent SDS-PAGE analysis of fusion protein expression. 1, 2: Total lysates of induced E. coli; 3: flowthrough of loaded proteins after purification; 4: proteins before purification; 5–9: various elutions of the 12T protein after purification. M indicates low molecular marker (Amersham). (B) Binding assay of 12T, scFv25-TSST-1 (DT), or TSST-1 alone to hepatoma cells under different concentrations (same starting concentration 3 µM, 5× serial dilution). (C) Binding assay of 12T to SMMC-7721, BGC823, KB versus normal liver cells by cell-ELISA assays under different concentrations (same starting concentration 3 µM, 5× serial dilution). The data are the means of triplicate samples, and the error bars indicate standard deviation.

Back to article page

Molecular Medicine

ISSN: 1528-3658