Figure 4

12T fusion protein inhibits tumor growth in vitro and in vivo. (A) Cytotoxic effects on the hepatoma cell line SMMC-7721 by 12T and TSST-1 in vitro. SMMC-7721 cells were incubated with human T cells, with either 12T or native TSST-1, which were diluted at the same starting concentration (10 nM) before the surviving tumor cells were measured by MTS assays. Effector:target ratio is 8:1. The data are the means of triplicate samples, and the error bars indicate standard deviation. (B) Cytotoxic effects of 12T on SMMC-7721 cells with different effector:target ratios. These were diluted at the same starting concentration (10 nM) before the surviving tumor cells were measured by MTS assays. The data are the means of triplicate samples, and the error bars indicate standard deviation. (C) 12T inhibits tumor growth in vivo. The tumor nodules were isolated and weighed after injecting different doses of 12T, TSST-1 alone, or a control. The inhibition rate of the experimental group was calculated versus the control. The data are the mean ± SD of 8 individual mice.