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Table 2 Semiquantitative analysis of mRNA for TGF-β3, TGFBR1, TGFBR2, TGFBR3, RARA, and GABRB3 in N-SP and CLP-SP fibroblasts treated or not with RA.

From: Retinoic Acid, GABA-ergic, and TGF-β Signaling Systems Are Involved in Human Cleft Palate Fibroblast Phenotype

 

N-SP fibroblasts

CLP-SP fibroblasts

 

Control

RA

Control

RA

TGF-β3

100 ± 13

242 ± 27d

143 ± 17b

228 ± 25d

TGFBR1

100 ± 13

65 ± 7d

103 ± 11c

57 ± 7d

TGFBR2

100 ± 11

267 ± 28d

111 ± 11c

285 ± 30d

TGFBR3

100 ± 12

95 ± 11NS

107 ± 13c

123 ± 14NS

RARA

100 ± 14

170± 19d

315 ± 35a

510 ± 54d

GABRB3

100 ± 11

307 ± 35d

540 ± 59a

590 ± 68NS

β-Actin

100 ± 11

95 ± 10NS

100 ± 12c

91 ± 11NS

  1. The values indicate mRNA levels corrected for β-actin mRNA levels and expressed as the percentage of untreated N-SP and CLP-SP fibroblasts. All values are mean ± SD of 4 separate experiments for each of the 4 patients; each experiment was performed in quadruplicate. The results were analyzed by ANOVA. CLP-SP control fibroblasts vs. N-SP control fibroblasts: aF test significant at 99%; bF test significant at 95%; cnot significant; treated vs. untreated control cells: dF test significant at 99%; NS, not significant.
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Molecular Medicine

ISSN: 1528-3658