Figure 2

MyD88 expression in the Kupffer cells from female and male mice. Three groups, untreated female and male mice and male mice treated with 17β-estradiol for 1 d (Male + E2), were subjected to hypoxia (HP) or normoxia (S), and then Kupffer cells were prepared. (A) A representative immunoblot showing MyD88 protein (34 kDa) levels in Kupffer cells of various groups. β-Actin was used as a protein loading control. (B) The expression of MyD88 protein was quantitatively analyzed densitometrically, and the densitometric values from 4 individual experiments were pooled and are presented as mean ± SE. (C) Real-time PCR for MyD88 transcript. The experiment was repeated 3 times and the results are presented as mean ± SE. P values are shown between 2 indicated groups.