Figure 3

Src expression in the Kupffer cells from female and male mice. Untreated female and male mice, plus a separate group of male mice treated with 17β-estradiol for 1 d (Male + E2), were subjected to hypoxia (HP) or normoxia (S), and Kupffer cells were isolated. Kupffer cells were lysed, and the lysates were analyzed for Src protein contents. (A) A representative immunoblot of Src proteins in the Kupffer cells and β-actin. (B) The expressions of Src protein were quantitatively analyzed densitometrically, and the densitometric values from 4 individual experiments were pooled and are presented as mean ± SE.