Figure 4

IL-6 mRNA expressions in Kupffer cells from male and female mice by realtime RT-PCR. Three groups, untreated female and male mice and male mice treated with 17β-estradiol for 1 d (Male + E2), were subjected to hypoxia (HP) or normoxia (S), and Kupffer cells were prepared. Kupffer cell RNA was isolated and real-time PCR was performed for IL-6 mRNAs. The experiment was repeated 3 times and the results are presented as mean ± SE. P values are shown between 2 indicated groups.