Figure 6

Effect of PP1 on Src phosphorylation and protein expression in the Kupffer cells from male mice following hypoxia. Male mice were treated intraperitoneally with Src inhibitor PP1 at a dose of 1.5 mg/kg body weight 1 h before hypoxia. Kupffer cells were isolated, lysed, and immunoblotted for Src phosphorylation and protein levels. (A) A representative immunoblot analysis of Src phosphorylation and protein levels in Kupffer cells. (B) The membranes were stripped and reprobed for β-actin for protein loading control. The blots were quantitatively analyzed by densitometry and the densitometric units from more than 4 animals in each group were pooled and are shown as mean ± SE. In addition, densitometric values for phosphorylation were normalized to the total protein. (C) Normalized phosphorylation is shown as mean ± SEM. P values are shown between 2 indicated groups.