Figure 5
From: Activation of the Poly(ADP-Ribose) Polymerase Pathway in Human Heart Failure
Detection of poly(ADP-ribose) polymerase (PARP-1) expression. Human ventricular tissue samples (F, failing; D, donor) were loaded (15 µg of protein in each lane) onto 10% SDS-polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with a PARP-1-specific antibody (1:10,000; Calbiochem) and also stained with Ponceau. In the densitometric analysis, the optical density of the band representing full-length PARP-1 (indicated) was corrected with the loading, and the mean values ± SEM are shown in the bar graph (donor, n = 5; failing, n = 7; P = 0.33).