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Table 1 Gene transfer with different pseudotyped MMLV, virosomes, and immunovirosomes.

From: Changing Viral Tropism Using Immunoliposomes Alters the Stability of Gene Expression: Implications for Viral Vector Design

 

Viral coat proteins

 

Amphotropic

Ecotropic

GALV

VSV-G

Unstimulated HSVECs

    

MMLV

0.3 ± 0.3

0.1 ± 0.1

0.7 ± 0.5

0.4 ± 0.8

MMLV + polybrene

9.2 ± 1.7

3.1 ± 2.9

10.2 ± 2.8

10.1 ± 2.3

Virosomes

19.1 ± 3.2

20.8 ± 2.4

22.8 ±3.1

21.8 ± 1.7

Anti-CD71 immunovirosomes

39.3 ± 3.4

40.8 ± 1.7

41.9 ± 6.9

42.7 ± 3.5

Stimulated HSVECs

    

MMLV

0.2 ± 0.1

0.3 ± 0.2

0.3 ± 0.1

0.4 ± 0.3

MMLV + polybrene

10.8 ± 0.3

2.8 ± 3.9

10.7 ± 3.4

10.6 ± 4.9

Virosomes

22.2 ± 8.1

22.7 ± 3.2

22.3 ± 4.5

24.1 ± 3.8

Anti-CD62E/P immunovirosomes

35.2 ± 1.8

36.9 ± 3.3

37.1 ± 5.2

38.1 ± 7.1

  1. HSVECs (either nonactivated or activated) were treated with amphotropic, ecotropic, GALV- and VSV-G-pseudotyped MMLV. These pseudotyped MMLV were then used to generated virosomes [Tfx-50, lipofectin (data not shown) or LipofectAMINE (data not shown)] and anti-CD71 containing immunovirosomes for nonactivated ECs (upper panel or anti-CD62E/P containing immunovirosomes for cytokine-activated ECs (lower panel). All results are expressed as mean ± SD of transfection efficiency of triplicate wells and all experiments were carried out at least three times.
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Molecular Medicine

ISSN: 1528-3658