Table 2 Primers and PCR conditions applied
target gene | primers name | sequence | annealing T° (°C) | MgCl2 (mM) | PCR cycles (n°) | product (bp) |
|---|---|---|---|---|---|---|
CASQ | CASQ-f CASQ-r | gcc atc ccc aac aaa cc ggc aac gag cag agg aaa gt | Â | Â | Â | 276 |
PLN | PLN-f PLN-r | aaa ctc ccc agc taa aca cc gaa ctt cag aga agc atc acg atg ata | 62 | 1,0 | 27 | 337 |
RyR2 | RyR2-f RYR2-r | cgt tct aac cag cat ctc atc cga gca ata caa cct gac c | 58 | 1,0 | 27 | 343 |
SERCA2a | SERCA2-f SERCA2-r | aca cca aat aaa cca agc ag ttt tag act tgt ggg agg gt | 56 | 1,0 | 27 | 564 |
SLN | SLN-f | gat cct ctt cag gag gtg ag | 60 | 1,0 | 27 | 568 |