Figure 1
From: Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell
YSL effects on the experimental lung metastasis model of B16-F10 cells. C57/BL6 mice (10 mice/group) received intravenous injection of B16-F10 melanoma cells (2 × 105 cells/mouse) and killed 20 d later. The lungs were collected and fixed in Bouin’s solution. The numbers of lung metastatic lesions were counted under a dissecting microscope. The results are expressed as the means ± SD. (a) Impact of YSL on the number of lung metastasis lesions of B16-F10 cells. *P< 0.05 vs. control (saline) group. Formalin-fixed, paraffin-embedded sections of each lung tissue were stained routinely with HE. Histopathology examination was completed using Olympus microscopy. (b) YSL 320 µg/kg per day: HE, ×40, nidulant lung metastatic lesions surrounded the pleura, blood vessel, or trachea vessel; (c) YSL 160 µg/kg/d: HE, ×40, nidulant lung metastatic lesions near the pleura; (d) saline: HE, ×40, nidulant lung metastatic lesions dispersed in whole lung tissue.