Figure 3

Inhibitory effect of YSL on B16-F10 cell adhesion to Matrigel. Ninety-six-well cell culture plates were coated with 25 mL Matrigel (200 µg/mL) and incubated at 37°C for 1 h and then washed with PBS. The coated wells were overlaid with 1% BSA for 1 h, washed with PBS, and dried at room temperature. Cells treated with YSL for 24, 48, or 72 h were applied to individual wells coated with Matrigel at 5 × 10⁵/mL and incubated for 1 h at 37°C in 5% CO₂. The A values of all wells were measured by the MTT method at 570 nm. The results are expressed as the inhibition of cell adhesion of 6 experiments: rate of inhibition (%) = (1 — mean A value of treatment group/mean A value of control) × 100. *P < 0.05 compared with control group.