Figure 4

Inhibitory effect of YSL on B16-F10 cell invasion. Polyvinylpyrrolidone-free polycarbonate filters with a pore size of 8 µm were coated with fibronectin on the lower surface. Reconstituted basement membrane Matrigel was added to the upper surface of the filter to form a matrix barrier. B16-F10 cells treated with various doses of YSL (0.01~100 µg/mL) and RPMI 1640 (control) for 24, 48, or 72 h were incubated in the upper compartment of a transwell culture chamber for 4 h. The numbers of invasion cells were determined as described previously. The result was expressed as follows: rate of inhibition (%) = (1 — number of invading cells of the treatment group/number of invading cells of control group) × 100. (A) Effect of YSL on the number of invading B16-F10 cells. *P< 0.05 compared with control group. (B) YSL 0.01 µg/mL, HE, ×200; (C) YSL 0.1 µg/mL, HE, ×200; (D) YSL 1 µg/mL, HE, ×200; (E) control group, HE, ×200.