Figure 5
From: Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell
Inhibitory effect of YSL on mRNA expression of ICAM-1. After B16-F10 cells were treated with YSL (0.01 or 0.1 µg/mL) or RPMI 1640 (control) for 48 h, mRNA was extracted and reverse transcribed to cDNA. The cDNA was subjected to realtime quantitative PCR using gene-specific primers for ICAM-1, and β-actin was used as reference. The relative amount of ICAM-1 was calculated using the 2−ΔCT equation. n = 3, mean ratio ± SD; *P< 0.05 compared with control group.