Proteome Analysis of Cultivated Vascular Smooth Muscle Cells from a CADASIL Patient

Table 1 Identified Proteins^a

Spot no.	Protein name	SwissProt Accession no.	Theoretical MW/pl	Sequence coverage	Probability score	ANOVA (P)	Change CADASIL/Control
Protein degradation and folding
7602	Proteasome component C3	P25787	25.8/7.1	20%	64	< 0.0001	1.6
3501	Proteasome β chain	P28070	26.0/5.6	42.1%	69	< 0.02	2.1
3701	Ubiquitin carboxyl-terminal esterase L1	P09936	24.9/5.2	43%	100	< 0.0002	0.3
2504	Heat shock protein 27	P04792	22.8/6.0	39.5%	84	< 0.03	1.7
Cytoskeleton interaction/SMC contraction
1605	Rho protein GDP-dissociation inhibitor	P52565	23.3/5.0	28%	90	< 0.000003	3.4
8203	Profilin 1	P07737	15.1/8.5	38%	111	< 0.004	6.3
1503	Cystein and glycine rich protein 1	P21291	21.3/8.9	64%	88	< 0.05	3.7
Free radical scavenger/cellular stress
8501	Superoxide dismutase. mitochodrial	P04179	24.9/8.3	33%	82	< 0.00004	2.0
3606	Glutathione S-transferase P1	P09211	23.4/5.3	53%	101	< 0.05	0.5
5803	Glutathione S-transferase omega	P78417	27.8/6.2	20%	66	< 0.03	0.4
Other
3401	Aspartyl-tRNA synthetase	P14868	57.5/6.1	14%	72	< 0.00002	5.7

The spot numbers are the same as in Figure 1. The accession number is Swiss-prot database accession number; theoretical molecular weight (MW) and isoelectric point (pl) for the proteins are obtained from Swiss-prot database. Probability scores and sequence coverage are from Mascot search engine, and probability scores >60 were considered as significant. The significance of the intensity differences was calculated with one-way ANOVA (P-values), and the change in the average spot intensity of the CADASIL sample is represented as comparison to the average spot intensity of the control sample (CADASIL/Control).

ISSN: 1528-3658