Figure 4
From: NFκB Is Persistently Activated in Continuously Stimulated Human Neutrophils
IκBα associates with NFκB in the nuclear matrix of human neutrophils. (A) Neutrophils were stimulated with LPS (100 ng/mL) for 0, 0.5, and 9 h and fractionated to prepare soluble (1), chromatin (2), chromatin wash (3), and nuclear matrix (4) fractions. The fractions were analyzed by Western blotting using LDH, histone H2A, lamin B, IκBα, and p50 and p65 NFκB antibodies. Each lane contains approximately 106 cells. (B) Neutrophils were stimulated with LPS (100 ng/mL) for 0, 0.5, and 9 h, and the nuclear extracts were immunoprecipitated on anti-IκBα-agarose. The immunoprecipitated proteins were detected by immunoblotting using IκBα and p65 NFκB antibodies.