Figure 5
From: Phosphorothioate Oligonucleotides Reduce PrPSc Levels and Prion Infectivity in Cultured Cells
PrP levels before and after the removal of 22-mer PS-DNA from ScN2a cells. ScN2a cells were exposed to various concentrations of PS-DNA for 2 weeks. Thereafter, the cells were grown for another week in DNA-free media, then harvested and digested with PK to determine levels of PrPSc. PrPC levels returned to normal levels whereas PrPSc levels were not restored after a recovery period of 1 week. Fab D18 was used to detect PrPC and PrPSc.