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Table 4 Effect of HF diet feeding on serum lipid peroxide levels (TBARS), PON1 activities, PON1, apoA-I and apoJ levels, and liver SR-BI levels.

From: Paraoxonase 1 Response to a High-Fat Diet: Gender Differences in the Factors Involved

 

Male

Female

 
 

Control

HF fed

Control

HF fed

ANOVA

Serum

     

TBARS (µmol/L)

1.40 ± 0.03

1.73 ± 0.19

1.53 ± 0.22

1.44 ± 0.09

NS

Paraoxonase activity (nKat/mL)

1.16 ± 0.09

0.96 ± 0.03

1.38 ± 0.06

1.01 ± 0.06

G,D

Arylesterase activity (µKat/mL)

2.34 ± 0.28

2.25 ± 0.17

2.70 ± 0.34

2.43 ± 0.28

NS

PON1(%)

100 ± 18

70 ± 20

218 ± 36

157 ± 17

G

ApoA-I (%)

100 ± 8

89 ± 18

152 ± 21b

60 ± 14a

D, G*D

ApoJ (%)

100 ± 13

111 ± 16

137 ± 16

87 ± 11a

G*D

Liver

     

SR-BI (%)

100 ± 5

73 ± 39

184 ± 34

159 ± 17

G

  1. aHF vs. control
  2. bfemale vs. male.
  3. Values are expressed as the mean ± S.E.M. of seven animals per group.
  4. Serum levels of PON1, apoA-I and apoJ, and liver levels of SR-BI were detected by immunoblot.
  5. Levels of control rats were set as 100%.
  6. ANOVA (P < 0.05).
  7. G, gender effect.
  8. D, diet effect.
  9. G*D, interactive effect.
  10. t-Test (P < 0.05).