Figure 2

A pathway diagram developed using the Ingenuity Pathways Analysis and Knowledge Base in which all genes changed by more than 1.5 fold were queried. This diagram shows one of the relational networks in which all up-regulated genes (green) were those related to astrocyte structure or function. This network is very significant with a score of 40, which means that the association by chance alone is less than 99.99%. Those genes with a significant increase in fold change in our study are shaded green, while those with a decrease are shaded in red. In the diagram, the vertical diamond shape represents an enzyme, a horizontal diamond is a peptidase, downward pointing triangle is a kinase, horizontal rectangle is a nuclear receptor, the oval a transcription factor, and the round other genes. Lines with arrow head = acts on; line with short perpendicular line at end = inhibits; plain line = binds only.

Abbreviations of gene names: ADAMTS3, Disintegrin-like and metalloprotease with thrombospondin type 1 motif, 3; AHNAK, AHNAK nucleoprotein (desmoyokin); CAPN2, Calpain 2; CAPN3, Calpain 3; CAST, Calpastatin; CD44, CD44 antigen (homing function and Indian blood group system); CRYAB, Crystallin α B; CSPG2, Chondroitin sulfate proteoglycan 2 (Versican); CSPG3, Chondroitin sulfate proteoglycan 3 (neurocan); FBXW7, F-box and WD-40 domain protein 7; FGF1, Fibroblast growth factor 1 (acidic); FGFR2, Fibroblast growth factor receptor 2; G3PB2, Ras-GTPase activating protein SH3 domain-binding protein 2; GFAP, Glial fibrillary acidic protein; KIAA0992, Palladin; MET, Met proto-oncogene (hepatocyte growth factor receptor); MSN, Moesin; NET1, Neuroepithelial cell transforming gene 1; NFKBIA, Nuclear factor kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; PCTK3, PCTAIRE protein kinase 3; PDE8A, Phosphodiesterase 8A; PLEC1, Plectin 1, intermediate filament binding protein 500kDa; PPFIA1, Protein tyrosine phosphatase, receptor type f, interacting protein (liprin), α 1; PSEN1, Presenilin 1 (Alzheimer disease 3); PTGS2, Prostaglandin-endoperoxide synthase 2 (G/H synthase and cyclooxygenase); RDX, Radixin; S100A6, S100 calcium binding protein A6 (calcyclin); S100B, S100 calcium binding protein, β (neural); TNC, Tenascin-C; TTN, Titin; VCAM1, Vascular cell adhesion molecule 1; VIL2, Villin 2 (ezrin); YWHAZ, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.