Figure 3

This figure shows photomicrographs of the localization in area CA1 pyramidal layer of non-sclerotic (non-MTLE, A, C. E. G, I) and sclerotic (MTLE, B, D, F, H, J) hippocampi of some proteins whose genes showed changes in expression in this study. A & B show the immunolocalization of AQP4 (Aquaporin 4). Note the reduction in immunoreactivity in astrocytic foot processes around blood vessels in MTLE and increase in astrocytic processes throughout the pyramidal layer. C & D show reduction in DMD (Dystrophin) around blood vessels in MTLE. E & F show localization of CD44 (CD44 antigen). In the non-sclerotic hippocampus (E) immunoreactivity is seen on fiber-like processes (arrow) extending into the pyramidal layer from the stratum oriens. Scattered among these fibers are CD44 positive astrocyte cell bodies (not shown). In MTLE, many more astrocytes are immunoreactive and form a dense network throughout the region with more intense staining in perivascular foot processes (arrow in F). G & H show PLEC1 (Pectin 1, intermediate filament binding protein 500kDa) immunoreactivity, which is increased in expression in astrocyte cell bodies and perivascular foot processes (arrow). I & J show an increase in CXCR4 (Chemokine (C-X-C motif) receptor 4) expression on microglia in the sclerotic hippocampus (arrow). CXCR4 is increased on a small proportion of astrocytes as well.