Figure 1
(A) The parental MCF-10F cell line was treated with 17β-estradiol (E2) 10−8 M, parathion (100 ng/mL) and combination of E2 plus parathion during 20 passages. AIA: Anchorage Independence Assay. IA: Invasion Assay. Positive signs (+): referred to the lack of anchorage independent growth and invasiveness. Negative signs (−): referred to the lack of both characteristics. (B) MCF-10F cell line did not form colonies in agar. (C) Colony formed by parathion-treated cells. (D) Colony formed by combination of E2- and parathion-treated cells. Phase contrast microscopy (4X). (E) Invasive characteristics of control and treated-MCF-10F cell line were scored 20 h after plating onto matrigel basement membranes using modified Boyden’s chambers constructed with multi-well cell culture plates and cell culture inserts. Values represent the invasive characteristics of MCF-10F, E2, parathion, and a combination of E2- and parathion-treated cells obtained by scoring the number of cells that crossed the filters.