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Figure 3 | Molecular Medicine

Figure 3

From: Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library

Figure 3

Evaluation by cell-ELISA of the binding selectivity of seven phage clones.. Cells were grown on 96-well plates overnight at the same concentration (1 ×105 cells/well). About 1×1010 pfu phages were added to each well at 37 °C for 2 h. Mouse anti-M13 bacteriophage antibody and HRP-conjugated sheep anti-mouse Ig were added in turn. OD490 was obtained after blocking of the reaction. The selectivity values of each phage clone (S1S20), calculated by the formula mentioned in the text, were 3.52, 3.18, 2.66, 3.01, 3.15, 3.49, and 2.55, respectively. Therefore clone S1 appeared to bind more effectively than the other clones.

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Molecular Medicine

ISSN: 1528-3658