Figure 4
HSP27 inhibits viral replication in CEM-SS and H9 T-lymphocytes in a Vpr-dependent manner. (A) HSP27 expression analysis in stably transfected cells by Western blot. Ctr — mock-transfected cells; lanes 1–3: H9 cells; lanes 4–6: CEM-SS cells; pcDNA3.1 — cells transfected with an empty vector; + HSP27 — cells transfected with HSP27-expressing plasmid. (B) Stable expression of HSP27 reduces viral replication in CD4 + H9 and CEM-SS cells in a Vpr-dependent manner. Viral replication was determined by measuring p24 antigen in culture supernatant at indicated time. *, indicates statistical significance (at the level of P< 0.01) between HIV-1NLHX-infected cells with or without overexpression of HSP27.