Figure 1

NOS expression in bone marrow cells. (A) Q-PCR analysis of NOS isoform expression. mRNA expression of each NOS isoform was determined in cultured stromal cells (SC) and hematopoietic cells (HC) using Q-PCR and isoform-specific primers. Data are presented as a ratio of expression level of NOS isoform in stromal cells to that in bone marrow. Data were analyzed using t test; *P < 0.005, **P < 0.0001, #P < 0.005. (B) Western blotting analysis of NOS isoform expression. Lysates of bone marrow (BM), primary bone marrow culture (PBMC), SC, HC, human endothelial cells (HE), and brain (BR) (latter two as control and reference) were analyzed by electrophoresis and blotting using antibodies against individual NOS isoforms and β-actin (loading control).