Figure 2
From: Neuronal Nitric Oxide Synthase Contributes to the Regulation of Hematopoiesis
NOS expression in the fetal liver. (A) Fetal liver is enriched in nNOS. RNA expression of NOS isoforms was examined by Q-PCR in liver at 14 days of embryonic development (e14) and postnatal day 1 (p1) and in adult liver. Data for each NOS isoform are presented as ratio of expression level of NOS isoform in e14 and p1 liver to that in adult liver. Data were analyzed using t test; *P < 0.05, **P < 0.005, #P < 0.005. (B) nNOS expression in the fetal liver. Expression of nNOS was determined in sections of e14 (a), p1 (b), and adult (c) liver using monoclonal antibodies against nNOS. (d, e) Negative controls (omitting primary antibody) for e14 (d) and adult (e) liver. (C) Western blotting analysis of NOS isoform expression in the liver. Lysates of fetal liver at embryonic day 14 (Fl-e14), fetal liver at perinatal day 1 (Fl-p1), adult liver (AL), human endothelial cells (HE), macrophage (M), and brain (BR) (latter three as control and reference) were analyzed by electrophoresis and blotting using antibodies against individual NOS isoforms.