Figure 3
From: Neuronal Nitric Oxide Synthase Contributes to the Regulation of Hematopoiesis
Expression of nNOS correlates with the supporting ability of stromal cell lines. (A) nNOS expression in a set of fetal liver-derived stromal cell lines with different supporting capability for hematopoietic stem cells. mRNA expression for each NOS isoform was determined by Q-PCR. Data are presented as a ratio of expression level of NOS isoform in a stromal line to that in murine bone marrow. Data were analyzed using t test; *P < 0.02. (B) Western blotting analysis of NOS expression in fetal liver-derived stromal cell lines. Lysates of stromal cells, bone marrow, and brain (as control and reference) were analyzed after electrophoresis using antibodies against NOS isoforms. (C) LTC-IC assay. The number of early hematopoietic progenitors was determined by cultivating hematopoietic cells on the AFT024 stromal cell layer for 4 weeks and replating in methylcellulose for colony formation. Data are presented as the number of LTC-IC per 1 × 104 bone marrow cells taken for analysis. Data were analyzed using t test; *P < 0.02 (D) nNOS expression in selected stromal cell lines. mRNA expression of NOS isoforms in several stromal lines differing in their ability to support hematopoietic stem cells and in cultured stromal cells from fetal liver (FLS) determined by Q-PCR. Data are presented as a ratio of expression level of NOS isoform in a line to that in murine bone marrow. Data were analyzed using t test; *P < 0.05, **P < 0.001.