Figure 5
From: Neuronal Nitric Oxide Synthase Contributes to the Regulation of Hematopoiesis
Hematopoietic progenitors and mesenchymal stem cells in the NOS knockouts. Number of CFCs (A), hematopoietic cells (B), and stromal cells (C) per culture after cultivation of bone marrow cells from wild-type (WT) and NOS-knockout mice and spleen cells from wild-type and nNOS knockouts. Data are presented as a ratio of colonies or cells per culture in knockouts to those in wild-type mice. Data were analyzed using t test; *P < 0.05. (D) Addition of SNAP during cultivation decreases the number of hematopoietic progenitors. Bone marrow cells were cultivated in the Myelocult media with or without 250 µM SNAP for the indicated number of days, and the number of committed progenitors was determined using the CFC assay. The data are presented as a ratio of colonies per 104 cells after cultivation to the number of colonies in the starting culture (input), i.e., determined at day 0. Similar results were obtained when the CFC number per culture was calculated. Data were analyzed using t test; *P < 0.01, **P < 0.001, #P < 0.01, ##P < 5 × 10−7. (E) Number of CFC-Fs per 0.5 × 106 cells in the bone marrow of wild-type and nNOS-knockout mice. Data were analyzed using t test; *P < 0.05.