Figure 1

(A) Leakage of ETP from liposomized formulations in surrounding PBS buffer. Liposomized formulations of ETP were incubated in PBS for 24 h and 48 h. Aliquots were taken from the supernatant to determine released ETP by HPLC as described in Materials and Methods. Data are mean ± SD of 3 independent experimental values. ^*P< 0.001 (Tufts-Lip-ETP) versus Lip-ETP at 24 h and 48 h. (B) Hemolysis of human erythrocytes induced by various formulations of ETP. Human erythrocytes were incubated with free ETP, Lip-ETP, and Tuft-Lip-ETP for 1 h at 37 °C as described in Materials and Methods. Data are mean ± SD of 3 independent experimental values. Various groups were compared by one-way ANOVA followed by Dunnett’s post hoc test. ^aP < 0.001 versus free ETP; ^bP < 0.001 versus Lip-ETP.