Figure 3

Decreased Rac activity inhibits cell migration. (A) Phase contrast micrographs of RA-FLS immediately and 21 h after wounding. RA-FLS (RA1) were pre-treated with 50 µM NSC23766 or control solution for 24 h and maintained in the presence or absence of 50 µM NSC23766 after wounding. Images of RA-FLS were taken at time 0 and 21 h after wounding. (B) Quantification of wound healing. RA-FLS (RA1) were treated with 50 µM NSC23766 (open squares) or control solution (closed squares) as described in (A). The migration distance was quantified as described in Materials and Methods. Shown are the mean values (± SEM) of eight measurements for each time point. Data are representative of two independent experiments. (C) Quantification of migration of control cells (solid bars) or cells treated with 50 µM NSC23766 (empty bars) after 21 h of various RA-FLS lines. *** = P < 0.001, two-tailed t test. Data shown are representative of two independent experiments.