Figure 5

Inhibition of RA-FLS proliferation and invasion by siRNA-mediated depletion of Rac1. (A) Rac1 siRNA inhibits Rac expression. RA-FLS were transfected with Rac1 siRNA by nucleoporation. Rac protein levels were determined by Western blotting of cell lysates at the indicated days. Tubulin levels are shown as loading control. (B) Rac1-directed siRNA inhibits RA-FLS invasion. Matrigel invasion assays were performed on day seven post-transfection. Shown is the mean (± range) of two independent experiments. (C) Rac1-directed siRNA inhibits RA-FLS proliferation. SRB cell proliferation assays were initiated four days post-transfection. Shown is the mean (± range) of two independent experiments. *** = p < 0.001, two-tailed t test