Figure 5

Differential expression of p50 and p65 in PBMC proteins from pregnant FHF patients (FHF1 and FHF2). From each patient, 30 µg of protein was separated in a 10% SDS-PAGE mini gel. After electrotransfer, the filters were consecutively incubated with p50 and p65 antibodies of NF-kB family. To confirm, equal protein loading, the filters were reincubated with a polyclonal Actin antibody. Lanes a, normal healthy pregnant women as disease controls (N); lane b and c, pregnant hepatitis virus infected FHF patients. The increase in expression in comparison to the normal control as revealed by densitometry analysis is indicated below each lane.