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Figure 2 | Molecular Medicine

Figure 2

From: Valproic Acid Sensitizes Chronic Lymphocytic Leukemia Cells to Apoptosis and Restores the Balance Between Pro- and Antiapoptotic Proteins

Figure 2

Impact of VPA on the viability of PBMCs from healthy donors. (A) PBMCs from 5 healthy donors and from CLL patients were isolated, and spontaneous apoptosis was assessed by FACS. The diagram shows the relative amounts of viable cells (annexin V−/PI−) monitored over 7 days. (B) The graphs display the decrease in viable cells (annexin V−/PI−) from healthy donors monitored over 7 days of treatment in the presence of absence of different VPA concentrations normalized to untreated cells. (C) VPA-dependent apoptosis at 1 mM VPA was compared in PBMCs from CLL patients and healthy donors. The diagram presents the relative amounts of viable cells (annexin V−/PI−) normalized to untreated cells. (D) The table shows the significances (P values) determined for VPA-dependent apoptosis induction in CLL cells versus PBMCs from healthy donors. The viability of CD3+ cells (E) and CD19+ cells (F) from healthy donors during VPA treatment was compared with similarly treated CLL cells.

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Molecular Medicine

ISSN: 1528-3658