Table 2 Delivery systems used for gene transfer into mammalian cells.

1)

Naked Plasmids

2)

Physical vectors

a) Microinjection

b) Particle bombardment (gene gun)

c) Electroporation

d) Sonoporation

e) Laser irradiation

f) Magnetofection

g) Hydroporation

3)

Chemical vectors

a) Calcium phosphate

b) DEAE dextran

c) Cationic polyplexes

- Polylysin vector

- Polycation

d) Cationic lipoplexes

- Liposomes

- Nonliposomal

e) Cationic bioplexes

4)

Biologic viral vectors

a) Retrovirus

b) Adenovirus

c) Adeno-associated virus

d) Lentivirus

e) Other virus

5)

Biologic nonviral vectors

- Human artificial chromosomes