Figure 1

Costimulation of human monocytes with TLR2 and TLR4 agonists results in the synergistic release of CXCL8. THP-1 cells (n = 6) were cotreated with LPS (0.001 and 0.01 µg/mL) and (A) FSL-1 (TLR2/6 agonist; 0.1 µg/mL) or (B) Pam₃CSK4 (TLR2/1 agonist; 0.01 µg/mL) for 24 h, after which CXCL8 was measured in the supernatant by specific ELISA. *P < 0.05 between the sum of CXCL8 levels from TLR2 and TLR4 agonist together and the actual value of TLR2 and TLR4 costimulation, using ANOVA followed by a Bonferroni posttest.