Figure 4

Effect of LPA on LPS-induced NFκB activation in vivo. CD-1 mice were intravenously injected with LPA (10 mg/kg) or vehicle PBS followed by intraperitoneal injection of LPS (25 mg/kg) or control PBS. After 1 h and 6 h, lung NFκB activation was examined by EMSA. Arrows indicate NFκB. (A) Representative gel of NFκB activation after 1 h LPS challenge. (B) Representative gel of NFκB activation after 6 h LPS challenge. (C) Densitometry analysis of NFκB activation. N = 3–4; *, P < 0.05 compared with control.