Figure 8

Effect of PI3 kinase pathways on anti-inflammatory effect of LPA. Peritoneal macrophages were pretreated with wortmannin (10–100 nM) for 1 h followed by incubation with LPS (10 µg/mL) with or without LPA (10 µM) for 18 h. LPS-induced TNFα production was examined by ELISA. Data represent means ± SE from six independent experiments. *, P < 0.05 compared with basal; #, P < 0.05 compared with LPS group; f, P < 0.05 compared with no pretreatment LPA/LPS group.