Figure 1From: Association of Urinary N-Domain Angiotensin I-Converting Enzyme with Plasma Inflammatory Markers and Endothelial FunctionGel filtration chromatography of human urine: 1.0 mL of concentrated urine from normotensive subjects (A): with family history of hypertension (FH+) and (B): without family history of hypertension (FH−) were submitted separately to gel filtration on an AcA-44 column equilibrated and eluted as described in the Methods section. Fractions of 1.0 mL were collected at a flow rate of 20 mL/h; (—) Optical Density (OD) 280 nm; (—) ACE activity on Z-PheHL as substrate.Back to article page